高效液相色谱-质谱联用测定大鼠全血中二甲基姜黄素的浓度

王凤飞,章晓茜,曹聪,申屠建中,唐岚

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (22) : 1996-1999.

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中国药学杂志 ›› 2015, Vol. 50 ›› Issue (22) : 1996-1999. DOI: 10.11669/cpj.2015.22.015
论 著

高效液相色谱-质谱联用测定大鼠全血中二甲基姜黄素的浓度

  • 王凤飞1,章晓茜2,曹聪3,申屠建中4*,唐岚1*
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Determination of ASC-J9 in Rat Blood by HPLC-MS/MS

  • WANG Feng-fei1, ZHANG Xiao-qian2, CAO Cong3, SHENTU Jian-zhong4*,TANG Lan1*
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摘要

目的 建立检测大鼠全血中ASC-J9的高效液相色谱-质谱联用(HPLC-MS/MS)。方法 方法全血样本经液液萃取提取分离,色谱柱为Symmetry C18(4.6 mm×150 mm,5 μm),流动相为纯水(含0.1%甲酸和10 mmol·L-1甲酸铵)-乙腈,流速为1.0 mL·min-1,质谱分流0.4 mL·min-1,柱温35 ℃,大气压电喷雾离子源(ESI),正离子模式多重反应选择离子检测(MRM)。结果 ASC-J9线性范围为3.54~1 180 ng·mL-1,提取回收率在83.19%~87.27%,日内、日间RSD均小于8.89%。结论 本方法专属性强,灵敏度高,适用于ASC-J9药动学研究。

Abstract

OBJECTIVE To establish an HPLC-MS/MS method for determination of ASC-J9 in rat blood. METHODS After liquid-liquid extraction, ASC-J9 was separated on a Symmetry C18colume, with mobile phase of acetonitrile-water containing 0.1% formic acid and 10 mmol·L-1 ammonium formate.The flow rate was 1.0 mL·min-1, mass shunt was 0.4 mL·min-1, and column temperature was main tained at 35 ℃. Quantification was performed in positive ion multiple-reaction-monitoring(MRM) mode. RESULTS The calibration curve of ASC-J9 had good linearity in the concentration range of 3.54-1 180 ng·mL-1. The extraction recovery rate was within 83.19% to 87.27%,and the intra-day and inter-day RSDs were both less than 8.89%. CONCLUSION This method is specific, sensitive and suitable for determination of ASC-J9 in rat blood.

关键词

二甲基姜黄素 / 高效液相色谱-质谱联用 / 血药浓度 / 药动学

Key words

ASC-J9 / HPLC-MS/MS / blood concentration / pharmacokinetics

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导出引用
王凤飞,章晓茜,曹聪,申屠建中,唐岚. 高效液相色谱-质谱联用测定大鼠全血中二甲基姜黄素的浓度[J]. 中国药学杂志, 2015, 50(22): 1996-1999 https://doi.org/10.11669/cpj.2015.22.015
WANG Feng-fei, ZHANG Xiao-qian, CAO Cong, SHENTU Jian-zhong,TANG Lan. Determination of ASC-J9 in Rat Blood by HPLC-MS/MS[J]. Chinese Pharmaceutical Journal, 2015, 50(22): 1996-1999 https://doi.org/10.11669/cpj.2015.22.015
中图分类号: R917   

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